Journal: International Journal of Oncology

Article Title: Disruption of IGF-1R signaling by a novel quinazoline derivative, HMJ-30, inhibits invasiveness and reverses epithelial-mesenchymal transition in osteosarcoma U-2 OS cells

doi: 10.3892/ijo.2018.4325

Figure Lengend Snippet: HMJ-30 inhibits the Ras/MAPK, PI3K/AKT, and NF-κB signaling pathways in human osteogenic sarcoma U-2 OS cells. (A) Protein levels of Ras, p-ERK, p-JNK and p-p38 were downregulated, as determined by western blotting. Expression of the key proteins in MAPK signaling, (B) p-ERK (p-p44/42) and (C) p-p38, were reconfirmed using ELISA assays. (D) The protein levels of PI3K (p85) and p-AKT were downregulated, as determined by western blot-ting. (E) The key protein in p-AKT was reconfirmed by ELISA assay. (F) Protein levels of IKK, p-IκB and NF-κB (p65) were downregulated, as determined by western blotting. (G) NF-κB binding activity was determined using an electrophoretic mobility shift assay. Inhibitors of p44/22 MAPK (U0126), p38 (SB203580), PI3K (wortmannin) and NF-κB (PDTC), as positive controls, reduced protein kinase levels. The data represents the mean ± standard deviation of three experiments. *** P<0.001 vs. control. HMJ-30, 6-fluoro-2-(3-fluorophenyl)-4-(cyanoanilino)quinazoline; MAPK, mitogen-activated protein kinase; PI3K, phosphoinositide 3-kinase; AKT, protein kinase B; NF-κB, nuclear factor-κB; p-, phosphorylated; ERK, extracelullar signal-regulated kinase; JNK, c-Jun amino-terminal kinase; PDTC, ammonium pyrrolidinedithiocarbamate.

Article Snippet: p-IGF-1R β (Tyr1131) (cat. no. 7302), p-p38 MAPK (Thr180/Tyr182) (cat. no. 7946), p-p44/42 MAPK (Thr202/Tyr204) (cat. no. 7177), and p-Akt (Thr308) (cat. no. 7252) assays were performed according to the manufacturer's protocols (PathScan Sandwich ELISA kits; Cell Signaling Technology, Inc.).

Techniques: Protein-Protein interactions, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Binding Assay, Activity Assay, Electrophoretic Mobility Shift Assay, Standard Deviation, Control

Journal: PLoS ONE

Article Title: Kaposi’s Sarcoma Associated Herpesvirus Encoded Viral FLICE Inhibitory Protein K13 Activates NF-κB Pathway Independent of TRAF6, TAK1 and LUBAC

doi: 10.1371/journal.pone.0036601

Figure Lengend Snippet: A. Co-immunoprecipitation assay showing NEMO is essential for mediating the interaction of K13 with IKK1 and IKK2. FLAG-tagged K13 was immunoprecipitated from WT and NEMO-deficient Jurkat cells using control (C) and FLAG (F) antibody beads and presence of co-immunoprecipitated IKK1, IKK2 and NEMO detected by immunoblotting. B. MEFs expressing K13-ER TAM were treated with 4OHT and phosphorylation status of IKK1, IKK2 and IκBα was determined using Pathscan phospho-IKK1 (Ser176/180), phospho-IKK2 (Ser177/181), phosphor-IκBα (Ser32) ELISA kits, respectively.

Article Snippet: The PathScan Phospho-IKKα (Ser176/180), Phospho-IKKβ (Ser177/181) sandwich ELISA Kits and PathScan Phospho-IκBα (Ser32) sandwich ELISA antibody pair (Cell Signaling, Danvers, MA) were used to detect endogenous levels of IKKα, IKKβ and IκBα proteins when phosphorylated at Ser176/180, Ser177/181 and Ser32, respectively.

Techniques: Co-Immunoprecipitation Assay, Immunoprecipitation, Control, Western Blot, Expressing, Phospho-proteomics, Enzyme-linked Immunosorbent Assay

Journal: Journal of Virus Eradication

Article Title: Human cytomegalovirus (CMV) in Africa: a neglected but important pathogen

doi:

Figure Lengend Snippet: Comparison of human CMV seroprevalence in different countries in Africa

Article Snippet: Nigeria (Ilorin) , 93.9% , Asymptomatic HIV-positive adults , 180 , IgG ELISA Kit (DIA.PRO, Diagnostic Bioprobes, Italy) , .

Techniques: Comparison, Enzyme-linked Immunosorbent Assay, Agglutination, Cell Counting, Diagnostic Assay, Virus, Immunofluorescence

Journal: Journal of Virus Eradication

Article Title: Human cytomegalovirus (CMV) in Africa: a neglected but important pathogen

doi:

Figure Lengend Snippet: Comparison of human CMV seroprevalence in different countries in Africa

Article Snippet: Nigeria (Kano) , 91.1% , Pregnant women , 180 , CMV IgG ELISA kit (Dialab, Austria) , .

Techniques: Comparison, Enzyme-linked Immunosorbent Assay, Agglutination, Cell Counting, Diagnostic Assay, Virus, Immunofluorescence

Journal: PLoS ONE

Article Title: Role of Phenylalanine and Valine 10 Residues in the Antimicrobial Activity and Cytotoxicity of Piscidin-1

doi: 10.1371/journal.pone.0114453

Figure Lengend Snippet: Effects of Pis-V10K and Pis-F2K/V10K on TLR2, TLR4, phospho-ERKs, phosphor-JNK, and phosphor-p38 MAPK. TLR2, TLR4, phospho-ERKs, phosphor-JNK, and phosphor-p38 MAPK protein levels were determined by western blot analysis using specific antibodies.

Article Snippet: The membrane was blocked by incubating with 5% bovine serum albumin (BSA) in TBST (25 mM Tris, 3 mM KCl, 140 mM NaCl, 0.1% Tween 20) for 1 h at RT, and then incubated with antibodies specific for toll-like receptor 2 (TLR2, 1∶2000; Cell Signaling Technology, Beverly, MA, USA), TLR4 (1∶2000; Cell Signaling Technology), phospho-p38 (1∶2000; Cell signaling Technology), phospho-ERK (1∶2000; Cell signaling Technology), phospho-JNK (1∶2000; Cell signaling Technology), and β-actin (1∶5000, Sigma-Aldrich, St. Louis, MO, USA).

Techniques: Western Blot

Journal: Journal of leukocyte biology

Article Title: Halofuginone inhibits NF-kappaB and p38 MAPK in activated T cells.

doi: 10.1189/jlb.0705409

Figure Lengend Snippet: Fig. 4. Effect of halofuginone on p38 MAPK phosphoryla- tion. Halofuginone inhibits p38 MAPK phosphorylation in anti-CD3-activated T cells. The inhibition is dose-dependent. The data are means ( SD) of one representative experiment out of two performed.

Article Snippet: Phosphorylation of p38 was determined using the Human Phospho-p38 (T180/Y182) DuoSet IC ELISA kit (R&D Systems), according to the manufacturer’s instructions.

Techniques: Phospho-proteomics, Inhibition